真人做爰48姿势视图片|少妇真人直播免费视频|日本真人做爰A片|真人裸体做爰视频|真人与拘做爰视频免费观看|真人与拘做受免费视频一

溶血磷脂酸酸性磷酸酶(ACP6)活性蛋白

Active Acid Phosphatase 6, Lysophosphatidic (ACP6)

LPAP; ACPL1; PACPL1; Acid phosphatase-like protein 1; Lysophosphatidic acid phosphatase type 6

  • 溶血磷脂酸酸性磷酸酶(ACP6)活性蛋白 產(chǎn)品包裝(模擬)
  • 溶血磷脂酸酸性磷酸酶(ACP6)活性蛋白 產(chǎn)品包裝(模擬)
  • APD088Hu01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Lysophosphatidic acid phosphatase type 6 (ACP6), is a 44-47 kDa monomeric member of the histidine acid phosphatase family of proteins. ACP6 is highly expressed in kidney, heart, small intestine, muscle, liver, prostate, testis, ovary and exists as two isoforms as a result of alternative splicing events. It plays a role in regulating lipid metabolism that hydrolyzes LPA to monoacylglycerol (MAG) and phosphate. Annexin V (ANXA5) binds to phospholipid?bilayers, forming two-dimensional crystals that block the?phospholipids?from availability for coagulation enzyme reactions. A functional binding ELISA assay was conducted to detect the interaction of recombinant human ACP6 and recombinant mouse ANXA5. Briefly, ACP6 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ANXA5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ACP6 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human ACP6 and recombinant mouse ANXA5 was shown in Figure 1, the EC50?for this effect is 0.08 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPD088Hu01 溶血磷脂酸酸性磷酸酶(ACP6)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD088Hu01 溶血磷脂酸酸性磷酸酶(ACP6)活性蛋白 Cell?culture;?Activity?Assays.
PAD088Hu01 溶血磷脂酸酸性磷酸酶(ACP6)多克隆抗體 WB; IHC; ICC; IP.
留言咨詢
临澧县| 孟连| 于田县| 五原县| 静安区| 西乡县| 黄大仙区| 若羌县| 泗阳县| 松桃| 惠水县| 文化| 辉南县| 双鸭山市| 淮安市| 营口市| 克东县| 霞浦县| 南和县| 巴青县| 方城县| 三河市| 剑阁县| 确山县| 镇沅| 三门县| 合山市| 辽阳市| 梁山县| 治多县| 抚顺县| 广东省| 西畴县| 怀远县| 繁昌县| 玉环县| 二连浩特市| 绵阳市| 色达县| 兴义市| 大姚县|