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抑肽酶(AP)活性蛋白

Active Aprotinin (AP)

BPTI; Trasylol; Pancreatic Trypsin Inhibitor; Basic protease inhibitor

  • 抑肽酶(AP)活性蛋白 產品包裝(模擬)
  • 抑肽酶(AP)活性蛋白 產品包裝(模擬)
  • APA968Po62.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

活性實驗

Aprotinin (AP) is a competitive serine protease inhibitor. Reversibly binds to and blocks the enzymatic active site. Inhibits a range of serine proteases including trypsin, chymotrypsin, kallikrein and plasmin. Inhibits cytopathogenic effect of SARS-CoV-2 and double-stranded RNA formation in SARS-CoV-2-infected cells. The activity of recombinant pig AP was measured by its ability to inhibit trypsin cleavage of a peptide substrate BAPNA in the assay buffer 200 mM Triethanolamine hydrochloride, 20 mM CaCl2, pH 7.8. The reaction was performed in adding 20 μl 4 mg/mL trypsin diluted by 1mM HCl to 160 μl assay buffer and 20 ul 0.85% (w/v) NaCl and start the reaction by adding 100?μl of 1mg/ml BAPNA. Include a substrate blank containing 160 μl assay buffer, 20 μl 1mM HCl, 20 ul 0.85% (w/v) NaCl and 100?μL of 1mg/ml substrate. Rapidly mixing at 25 ℃, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader controlling the ?A405nm/min=0.08-0.12. The 20 ul different concentrations of recombinant pig AP was incubated with 20 ul 4 mg/mL trypsin in 160 ul assay buffer at 25℃ for 10?minutes followed by adding 100 ul substrate, then read at 405 nm in kinetic mode for 5 minutes using a microplate reader. Under these conditions, the enzyme amount of 50% inhibition of trypsin activity per minute is defined as a unit. The specific activity of recombinant pig AP is >600 U/mg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。

相關產品

編號 適用物種:Sus scrofa; Porcine (Pig,豬) 應用(僅供研究使用,不用于臨床診斷!)
APA968Po62 抑肽酶(AP)活性蛋白 Cell?culture;?Activity?Assays.
RPA968Po02 抑肽酶(AP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA968Po03 抑肽酶(AP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPA968Po61 抑肽酶(AP)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA968Po61 抑肽酶(AP)活性蛋白 Cell?culture;?Activity?Assays.
EPA968Po62 抑肽酶(AP)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA968Po01 抑肽酶(AP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA968Po01 抑肽酶(AP)多克隆抗體 WB; IHC; ICC; IP.
MAA968Po21 抑肽酶(AP)單克隆抗體 WB; IHC; ICC; IP.
CEA968Po 抑肽酶(AP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA968Po 抑肽酶(AP)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
24 Aprotinin prevents proteolytic epithelial sodium channel (ENaC) activation and volume retention in nephrotic syndrome. [pubmed:29042083]
Aus der Medizinischen Universit?tsklinik diss_Menacher word []
Acta Physiologica Plasminogen deficiency does not prevent sodium retention in a genetic mouse model of experimental nephrotic syndrome [Pubmed: 32455507]
Evaluation of The Relationship Between Apelin 36 and Oxidative Stress In Patients With General Anxiety Disorder []
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