Aryl Hydrocarbon Receptor (AhR), a protein of substantial molecular mass, typically ranging from 110 to 150 kilodaltons, is a member of the esteemed Basic Helix-loop-helix PAS (Per-Arnt-Sim) family of transcription factors. Ubiquitously expressed in vertebrate cells, AhR's activity is meticulously modulated by a variety of ligands. This receptor is pivotal in orchestrating gene transcription, thereby wielding considerable influence over a multitude of biological processes.Aryl Hydrocarbon Receptor Interacting Protein (AIP)can combine with AHR to regulate the endocrine system.Thus a functional ELISA assay was conducted to detect the interaction of recombinant recombinant mouse AhR and recombinant human AIP. Briefly, AhR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to AIP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-AhR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant recombinant mouse AhR and recombinant human AIP was shown in Figure 1, the EC50?for this effect is 0.49ug/mL.