碳酸酐酶Ⅸ(CA9)活性蛋白
Active Carbonic Anhydrase IX (CA9)
CA-IX; CAIX; MN; pMW1; P54/58N; Carbonic Dehydratase; Renal cell carcinoma-associated antigen G250; RCC-Associated Protein G250; Carbonate dehydratase IX
- 編號(hào)APD076Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)4.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Carbonic Anhydrase IX (CA9) also known as membrane antigen MN and renal cell carcinoma (RCC) associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. rhCA9 corresponds to the extracellular portion of human CA9. It catalyzes the reversible reaction of CO2 H2O = HCO3- H , which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. The recombinant human CA9 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (Assay Buffer), initiated by addition 50 μL of various concentrations of CA9 (diluted by Assay Buffer) to 50 μL of 2 mM Substrate 4-NPA (100 mM stock in Acetone, diluted by deionized water). Incubated at 37℃ for 5min, then read at a wavelength of 400 nm.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| EPD076Hu61 | 碳酸酐酶Ⅸ(CA9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD076Hu01 | 碳酸酐酶Ⅸ(CA9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APD076Hu61 | 碳酸酐酶Ⅸ(CA9)活性蛋白 | Cell?culture;?Activity?Assays. |
| APD076Hu01 | 碳酸酐酶Ⅸ(CA9)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAD076Hu01 | 碳酸酐酶Ⅸ(CA9)多克隆抗體 | WB; IHC; ICC; IP. |
| PAD076Hu06 | 碳酸酐酶Ⅸ(CA9)多克隆抗體 | WB; IHC; ICC; IP. |
| LAD076Hu71 | 碳酸酐酶Ⅸ(CA9)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAD076Hu21 | 碳酸酐酶Ⅸ(CA9)單克隆抗體 | WB; IHC; ICC; IP. |
| SED076Hu | 碳酸酐酶Ⅸ(CA9)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD076Hu | 碳酸酐酶Ⅸ(CA9)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
