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補體成分1q子成分A(C1qA)活性蛋白

Active Complement Component 1, Q Subcomponent A (C1qA)

Complement Component 1,q Subcomponent,Alpha Polypeptide

  • 補體成分1q子成分A(C1qA)活性蛋白 產(chǎn)品包裝(模擬)
  • 補體成分1q子成分A(C1qA)活性蛋白 產(chǎn)品包裝(模擬)
  • 補體成分1q子成分A(C1qA)活性蛋白 Figure . Gene Sequencing (extract)
  • APD207Hu01.png Figure. SDS-PAGE
  • 補體成分1q子成分A(C1qA)活性蛋白 Figure. Western Blot
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

Figure. The binding activity of C1qA with CRP.
The complement component 1q (C1q) is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Complement Component 1, Q Subcomponent A (C1qA) is six A-chains of C1q. Complement component 1q (C1q is a protein complex involved in the complement system, which is part of the innate immune system. C1q together with C1r and C1s form the C1 complex. It is potentially multivalent for attachment to the complement fixation sites of immunoglobulin. The sites are on the CH2 domain of IgG and, it is thought, on the CH4 domain of IgM. IgG4 cannot bind C1q, but the other three IgG types can. Besides, C Reactive Protein (CRP) has been identified as an interactor of C1qA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human C1qA and recombinant human CRP. Briefly, C1qA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to CRP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C1qA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of C1qA and CRP was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPD207Hu01 補體成分1q子成分A(C1qA)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APD207Hu01 補體成分1q子成分A(C1qA)活性蛋白 Cell?culture;?Activity?Assays.
PAD207Hu01 補體成分1q子成分A(C1qA)多克隆抗體 WB; IHC; ICC; IP.
MAD207Hu21 補體成分1q子成分A(C1qA)單克隆抗體 WB; IHC; ICC; IP.
SED207Hu 補體成分1q子成分A(C1qA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD207Hu 補體成分1q子成分A(C1qA)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSD207Hu01 補體成分1q子成分A(C1qA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
J Cell Mol Med LCN2 is a new diagnostic biomarker and potential therapeutic target in idiopathic short stature [Pubmed:35610759]
Journal of Biological Chemistry Competitive inhibition of the classical complement pathway using exogenous single-chain C1q recognition proteins [Pubmed:35690144]
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