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補體成分1q子成分B(C1qB)活性蛋白

Active Complement Component 1, Q Subcomponent B (C1qB)

Complement Component 1,q Subcomponent,Beta Polypeptide

  • 補體成分1q子成分B(C1qB)活性蛋白 產(chǎn)品包裝(模擬)
  • 補體成分1q子成分B(C1qB)活性蛋白 產(chǎn)品包裝(模擬)
  • APD208Hu01.jpg Figure. SDS-PAGE
  • 補體成分1q子成分B(C1qB)活性蛋白 Figure. Western Blot
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

C1qB protein is a component of the C1 complex in the classical pathway of the complement system. It is a subunit of the C1q molecule, which is the first component of the complement system to initiate the classical pathway. C1q is a hexameric protein composed of six identical subunits, each containing a C1qB, a C1qB, and a C1qC subunit. C1qB plays a key role in eliminating pathogens, regulating inflammatory response and maintaining immune tolerance. The binding of C4b and C1qB is a key step in the activation of the classical pathway in the complement system, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C1qB and recombinant mouse C4b. Briefly, C1qB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C4b-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C1qB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C1qB and recombinant mouse C4b was shown in Figure 1, the EC50?for this effect is 0.12 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
APD208Hu01 補體成分1q子成分B(C1qB)活性蛋白 Cell?culture;?Activity?Assays.
RPD208Hu01 補體成分1q子成分B(C1qB)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAD208Hu01 補體成分1q子成分B(C1qB)多克隆抗體 WB; IHC; ICC; IP.
SED208Hu 補體成分1q子成分B(C1qB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD208Hu 補體成分1q子成分B(C1qB)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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