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補(bǔ)體成分1q子成分C(C1qC)活性蛋白

Active Complement Component 1, Q Subcomponent C (C1qC)

C1QG; Complement Component 1 q subcomponent,Gamma Polypeptide

  • 補(bǔ)體成分1q子成分C(C1qC)活性蛋白 產(chǎn)品包裝(模擬)
  • 補(bǔ)體成分1q子成分C(C1qC)活性蛋白 產(chǎn)品包裝(模擬)
  • APE869Hu01.png Figure. SDS-PAGE
  • 補(bǔ)體成分1q子成分C(C1qC)活性蛋白 Figure. Western Blot
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Figure. The binding activity of C1qC with APOA1.
The complement component 1q (C1q) is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Complement Component 1, Q Subcomponent C (C1qC) is six C-chains of C1q. Complement component 1q (C1q is a protein complex involved in the complement system, which is part of the innate immune system. C1q together with C1r and C1s form the C1 complex. It is potentially multivalent for attachment to the complement fixation sites of immunoglobulin. The sites are on the CH2 domain of IgG and, it is thought, on the CH4 domain of IgM. IgG4 cannot bind C1q, but the other three IgG types can. Besides, Apolipoprotein A1 (APOA1) has been identified as an interactor of C1qC, thus a binding ELISA assay was conducted to detect the interaction of recombinant human C1qC and recombinant human APOA1. Briefly, C1qC were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to APOA1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C1qC pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of C1qC and APOA1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPE869Hu01 補(bǔ)體成分1q子成分C(C1qC)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APE869Hu01 補(bǔ)體成分1q子成分C(C1qC)活性蛋白 Cell?culture;?Activity?Assays.
PAE869Hu01 補(bǔ)體成分1q子成分C(C1qC)多克隆抗體 WB; IHC; ICC; IP.
MAE869Hu21 補(bǔ)體成分1q子成分C(C1qC)單克隆抗體 WB; IHC; ICC; IP.
SEE869Hu 補(bǔ)體成分1q子成分C(C1qC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCE869Hu 補(bǔ)體成分1q子成分C(C1qC)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LME869Hu 補(bǔ)體成分1q子成分C(C1qC)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
PLOS ONE Increased complement C1q level marks active disease in human tuberculosis [Pubmed:24647646]
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