纖維蛋白原β(FGB)活性蛋白
Active Fibrinogen Beta Chain (FGB)
FG-B; Fibrinopeptide B; Fibrinogen Beta
- 編號(hào)APB215Hu01
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)7.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1260 ¥ 3150 ¥ 6300 ¥ 18900 ¥ 47250
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Fibrinogen Beta Chain (FGB) is the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Fibrinogen Alpha Chain (FGA) has been identified as an interactor of FGB. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGB and recombinant mouse FGA. Briefly, FGB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FGA-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FGB and recombinant mouse FGA was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB215Hu01 | 纖維蛋白原β(FGB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB215Hu01 | 纖維蛋白原β(FGB)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB215Hu03 | 纖維蛋白原β(FGB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB215Hu02 | 纖維蛋白原β(FGB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB215Hu03 | 纖維蛋白原β(FGB)多克隆抗體 | WB; ICC; IHC-P; IHC-F; ELISA; FCM |
| PAB215Hu01 | 纖維蛋白原β(FGB)多克隆抗體 | WB; ICC; IHC-P; IHC-F; ELISA; FCM |
| PAB215Hu02 | 纖維蛋白原β(FGB)多克隆抗體 | WB,IHC; FCM |
| LAB215Hu71 | 纖維蛋白原β(FGB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAB215Hu81 | 纖維蛋白原β(FGB)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAB215Hu23 | 纖維蛋白原β(FGB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB215Hu25 | 纖維蛋白原β(FGB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB215Hu22 | 纖維蛋白原β(FGB)單克隆抗體 | WB; IHC |
| MAB215Hu24 | 纖維蛋白原β(FGB)單克隆抗體 | WB,IHC |
| MAB215Hu21 | 纖維蛋白原β(FGB)單克隆抗體 | WB,IHC |
| FAB215Hu06 | 抗纖維蛋白原β(FGB)單克隆抗體 | Flow cytometry. |
| FAB215Hu02 | 抗纖維蛋白原β(FGB)單克隆抗體 | Flow cytometry. |
| FAB215Hu04 | 抗纖維蛋白原β(FGB)單克隆抗體 | Flow cytometry. |
| FAB215Hu08 | 抗纖維蛋白原β(FGB)單克隆抗體 | FCM |
| LAB215Hu72 | 纖維蛋白原β(FGB)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAB215Hu82 | 纖維蛋白原β(FGB)單克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| SEB215Hu | 纖維蛋白原β(FGB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB215Hu | 纖維蛋白原β(FGB)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB215Hu01 | 纖維蛋白原β(FGB)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer [y2016:0077095.html] |
| Microbial Pathogenesis | Crystal structure of GAPDH of Streptococcus agalactiae and characterization of its interaction with extracellular matrix molecules [Pubmed: 30553015] |
| GLYCOCONJUGATE JOURNAL | Nattokinase-heparin exhibits beneficial efficacy and safety—an optimal strategy for CKD patients on hemodialysis [Pubmed: 30788657] |
