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成纖維細(xì)胞生長因子17(FGF17)活性蛋白

Active Fibroblast Growth Factor 17 (FGF17)

  • 成纖維細(xì)胞生長因子17(FGF17)活性蛋白 產(chǎn)品包裝(模擬)
  • 成纖維細(xì)胞生長因子17(FGF17)活性蛋白 產(chǎn)品包裝(模擬)
  • APC910Hu01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

Fibroblast?growth?factor?17(FGF17)??is?a?member?of?the?fibroblast?growth?factorfamily.?FGF?family?members?possess?broad?mitogenicand?cell?survival?activities?and?are?involved?in?a?varietyof?biological?processes,?including?embryonicdevelopment?cell?growth,?morphogenesis,?tissue?repair,?tumor?growth,?andinvasion. A proliferation assay was conducted to detect the bioactivity of recombinant human FGF17 using Balb/c 3T3 cells. Briefly,?3T3 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of FGF17 diluted with serum-free standard DMEM. After incubated for 48h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Balb/c 3T3 cells after incubation with FGF17 for 48h observed by inverted microscope was shown in Figure1. Cell viability was assessed by CCK-8(Cell Counting Kit-8 ) assay after incubation with recombinant FGF17 for 48h. The result was shown in Figure2. It was obvious that FGF17 significantly increased cell viability of 3T3 cells. The ED50 of recombinant human FGF17 is 335ng/ml.

Figure 2. The dose-effect curve of FGF17 on Balb/c 3T3 cells.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPC910Hu01 成纖維細(xì)胞生長因子17(FGF17)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC910Hu02 成纖維細(xì)胞生長因子17(FGF17)活性蛋白 Cell?culture;?Activity?Assays.
RPC910Hu02 成纖維細(xì)胞生長因子17(FGF17)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC910Hu01 成纖維細(xì)胞生長因子17(FGF17)活性蛋白 Cell?culture;?Activity?Assays.
PAC910Hu01 成纖維細(xì)胞生長因子17(FGF17)多克隆抗體 WB; IHC; ICC; IP.
MAC910Hu21 成纖維細(xì)胞生長因子17(FGF17)單克隆抗體 WB; IHC; ICC; IP.
SEC910Hu 成纖維細(xì)胞生長因子17(FGF17)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC910Hu 成纖維細(xì)胞生長因子17(FGF17)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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