成纖維細胞生長因子3(FGF3)活性蛋白
Active Fibroblast Growth Factor 3 (FGF3)
HBGF3; INT2; Heparin-binding growth factor 3; Murine Mammary Tumor Virus Integration Site(v-int-2)Oncogene Homolog; INT-2 Proto-Oncogene Protein; Proto-oncogene Int-2
- 編號APC906Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點11.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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WB圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Fibroblast Growth Factor 3 (FGF3) also known as INT-2 proto-oncogene protein is a member of the fibroblast growth factor family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Besides, Fibroblast Growth Factor Receptor 4 (FGFR4) has been identified as an interactor of FGF3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF3 and recombinant human FGFR4. Briefly, biotin-linked FGF3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGF3 and recombinant human FGFR4 was shown in Figure 1, the EC50?for this effect is 0.52 ug/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC906Hu01 | 成纖維細胞生長因子3(FGF3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC906Hu01 | 成纖維細胞生長因子3(FGF3)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC906Hu02 | 成纖維細胞生長因子3(FGF3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC906Hu01 | 成纖維細胞生長因子3(FGF3)多克隆抗體 | WB; IHC; ICC; IP. |
| LAC906Hu71 | 成纖維細胞生長因子3(FGF3)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC906Hu22 | 成纖維細胞生長因子3(FGF3)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC906Hu | 成纖維細胞生長因子3(FGF3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCC906Hu | 成纖維細胞生長因子3(FGF3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMC906Hu | 成纖維細胞生長因子3(FGF3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Mol Med Rep | Screening a novel FGF3 antagonist peptide with anti-tumor effects on breast cancer from a phage display library [PubMed: 26323695] |
