半乳糖凝集素2(GAL2)活性蛋白
Active Galectin 2 (GAL2)
LGALS2; HL14; Lectin,Galactoside-Binding Soluble 2; Beta-galactoside-binding lectin L-14-II; Lactose-binding lectin 2; S-Lac lectin 2
- 編號(hào)APA302Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份磷酸鹽緩沖液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Galectin 2 (GAL2) belongs to the proto type group and consists of two homologous carbohydrate recognition domains (CRDs) resulting in multiple sugar binding sites. The expression of gal-2 has been shown to be involved in processes of angiogenesis and inflammation but was not analyzed before in preeclamptic (PE) placentas.It also can agglutinate red blood. In this case, chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant mouse GAL2 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL2 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL2, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL2 is 5 μg/ml.
Figure 2. The hemagglutination assay of GAL2 in V- bottom shaped 96-well microtiter plate.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA302Mu01 | 半乳糖凝集素2(GAL2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA302Mu01 | 半乳糖凝集素2(GAL2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA302Mu01 | 半乳糖凝集素2(GAL2)多克隆抗體 | WB; IHC; ICC; IP. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biol Pharm Bull | Potential Interaction between Galectin-2 and MUC5AC in Mouse Gastric Mucus [Pubmed: 32009121] |
| International Journal of Molecular Sciences | Galectin-2 Has Bactericidal Effects against Helicobacter pylori in a β-galactoside-Dependent Manner [Pubmed: 32295066] |
