顆粒酶B(GZMB)活性蛋白
Active Granzyme B (GZMB)
GZM-B; HLP; CTLA1; CCPI; CGL1; CSP-B; CSPB; CTSGL1; SECT; Granzyme 2; Cytotoxic T-Lymphocyte-Associated Serine Esterase 1; Fragmentin 2; Cytotoxic Serine Protease B
- 編號APA600Po01
- 物種Sus scrofa; Porcine (Pig，豬) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)10.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1608 ¥ 4020 ¥ 8040 ¥ 24120 ¥ 60300
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T?lymphocytes (CTL) and natural killer (NK) cells. Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections. It activates various caspases and cleaves proteins such as aggrecan. The activity of recombinant pig Granzyme B was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl?HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (Assay Buffer), initiated by addition 50 μL of various concentrations of GZMB (diluted by Assay Buffer) to 50 μL of 1.2 mM Substrate and DTNB mixture. The final well serves as a negative control with no GZMB, replaced with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant pig Granzyme B is >440 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
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| 編號 | 適用物種：Sus scrofa; Porcine (Pig，豬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA600Po01 | 顆粒酶B(GZMB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA600Po01 | 顆粒酶B(GZMB)活性蛋白 | Cell?culture;?Activity?Assays. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Cancer | Effective immunotherapy of rat glioblastoma with prolonged intratumoral delivery of exogenous heat shock protein Hsp70 [Pubmed:24691976] |
| Biol Pharm Bull. | Z-100, an Immunomodulatory Extract of Mycobacterium tuberculosis Strain Aoyama B, Prevents Spontaneous Lymphatic Metastasis of B16-BL6 Melanoma [Pubmed:24818259] |
| Journal of Dermatological Science | Relationships?among?plasma?granzyme?B?level,?pruritus?and?dermatitis?in?patients?with?atopicdermatitis. [pubmed:27686401] |
| Oncotarget | Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment [Pubmed:29983866] |
| Theranostics | A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors [Pubmed: 31695790] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237] |
