顆粒酶K(GZMK)活性蛋白
Active Granzyme K (GZMK)
GZM-K; TRYP2; PRSS; Serine Protease,Granzyme 3; Tryptase II; Fragmentin-3; Granzyme-3; NK-tryptase-2
- 編號(hào)APB209Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)9.5
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Granzyme K is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T?lymphocytes (CTL) and natural killer (NK) cells. Human granzyme K is synthesized as a precursor (264?residues) with a signal peptide (residues 1 24), a propeptide (residues 25-26) and a mature chain (residues 27-264). The purified recombinant human Granzyme K consists of residues 27 to 264 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl?HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMK (diluted by assay buffer) to 50 μL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMK, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme K is >220 pmol/min/μg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB209Hu01 | 顆粒酶K(GZMK)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB209Hu01 | 顆粒酶K(GZMK)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB209Hu01 | 顆粒酶K(GZMK)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB209Hu81 | 顆粒酶K(GZMK)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB209Hu71 | 顆粒酶K(GZMK)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB209Hu21 | 顆粒酶K(GZMK)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB209Hu22 | 顆粒酶K(GZMK)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB209Hu | 顆粒酶K(GZMK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB209Hu | 顆粒酶K(GZMK)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Immunobiology. | Granzyme M and K release in human experimental endotoxemia [science:S0171298516300225] |
