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歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)活性蛋白

Active Homing Associated Cell Adhesion Molecule (HCAM)

CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8

  • 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)活性蛋白 產(chǎn)品包裝(模擬)
  • 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)活性蛋白 產(chǎn)品包裝(模擬)
  • APA670Ra01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Homing Associated Cell Adhesion Molecule (HCAM), also known as CD44, is a ubiquitous multistructural and multifunctional cells surface adhesion molecule involved in cell-cell and cell-matrix interactions. CD44 is broadly expressed, including in the membranes of B cells, granulocytes,?monocytes, and erythrocytes as well as on many?thymocytes?and mature?T cells, besides it is highly expressed in many cancers and regulates metastasis via recruitment of CD44 to the cell surface. This protein is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat HCAM and biotinylated hyaluronan (HA). Briefly, biotin-linked HA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HCAM-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 1 hour, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μl stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat HCAM and biotinylated HA was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA670Ra01 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA670Ra01 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)活性蛋白 Cell?culture;?Activity?Assays.
PAA670Ra01 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)多克隆抗體 WB; IHC
MAA670Ra21 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)單克隆抗體 WB; IHC; ICC; IP.
SEA670Ra 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA670Ra 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA670Ra01 歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It(ELISA Kit) Yourself"

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Cancers Predictive Value of Soluble PD-1, PD-L1, VEGFA, CD40 Ligand and CD44 for Nivolumab Therapy in Advanced Non-Small Cell Lung Cancer: A Case-Control Study [Pubmed: 32085544]
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