白介素10受體β(IL10Rb)活性蛋白
Active Interleukin 10 Receptor Beta (IL10Rb)
CDW210b; CD210-B; IL10R-B; IL10-RB; CRF2-4; CRFB4; IL-10R2; Interleukin-10 receptor subunit 2
- 編號APB638Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 97%
- 等電點5.0
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價格 ¥ 1272 ¥ 3180 ¥ 6360 ¥ 19080 ¥ 47700
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產品包裝(模擬)
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產品包裝(模擬)
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Figure. SDS-PAGE; Sample: Active recombinant IL10Rb, Human.
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Figure. Western Blot; Sample: Recombinant IL10Rb, Human.
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通過ISO 9001、ISO 13485質量體系認證
活性實驗
IL10Rb (Interleukin-10 receptor subunit beta) belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex, which is a cell surface receptor required for the activation of several cytokines, including IL10. Thus, a binding ELISA assay was conducted to detect the interaction of IL10Rb and IL10. Briefly, recombinant human IL10RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL10-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL10Rb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL10Rb and IL10 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
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| EPB638Hu61 | 白介素10受體β(IL10Rb)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB638Hu01 | 白介素10受體β(IL10Rb)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB638Hu01 | 白介素10受體β(IL10Rb)多克隆抗體 | WB; IHC; ICC; IP. |
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| MAB638Hu22 | 白介素10受體β(IL10Rb)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB638Hu | 白介素10受體β(IL10Rb)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB638Hu | 白介素10受體β(IL10Rb)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
