白介素17受體A(IL17RA)活性蛋白
Active Interleukin 17 Receptor A (IL17RA)
CDw217; CD217; IL17R-A; IL17-RA; IL17R-A; IL17R; HIL-17R
- 編號APB644Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1152 ¥ 2880 ¥ 5760 ¥ 17280 ¥ 43200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant IL17RA, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Interleukin-17 receptor A (IL17RA) is a cytokine receptor which binds interleukin 17A (IL17A). IL17A is a proinflammatory cytokine secreted by activated T-lymphocytes. IL17RA, as a transmembrane protein, is a ubiquitous type I membrane glycoprotein that binds IL17A, IL17F and IL17C. Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RA with recombinant human IL17A. Briefly, IL17RA were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17A-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RA with IL17A was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB644Hu02 | 白介素17受體A(IL17RA)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB644Hu01 | 白介素17受體A(IL17RA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB644Hu02 | 白介素17受體A(IL17RA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB644Hu01 | 白介素17受體A(IL17RA)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB644Hu02 | 白介素17受體A(IL17RA)多克隆抗體 | WB; IHC; ICC; IP. |
| RAB644Hu21 | 白介素17受體A(IL17RA)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| SEB644Hu | 白介素17受體A(IL17RA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB644Hu | 白介素17受體A(IL17RA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| IMMUNOBIOLOGY | The Role of IL-17A/IL-17RA and Lung Injuries in Children with Lethal Non-Pandemic Acute Viral Pneumonia [Pubmed: 32747026] |
