白介素6受體(IL6R)活性蛋白
Active Interleukin 6 Receptor (IL6R)
CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80
- 編號(hào)APB815Mu02
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)6.5
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1200 ¥ 3000 ¥ 6000 ¥ 18000 ¥ 45000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE; Sample: Active recombinant IL6R, Mouse.
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Figure. Western Blot; Sample: Recombinant IL6R, Mouse.
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
IL6R (Interleukin-6 receptor subunit alpha) is part of the receptor for interleukin 6. The interaction of IL6R and IL6 may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Thus, a binding ELISA assay was conducted to detect the association of IL6R with IL6. Briefly, IL6R were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL6-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL6R pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL6R with IL6 was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB815Mu02 | 白介素6受體(IL6R)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB815Mu02 | 白介素6受體(IL6R)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB815Mu01 | 白介素6受體(IL6R)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB815Mu02 | 白介素6受體(IL6R)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB815Mu01 | 白介素6受體(IL6R)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB815Mu71 | 白介素6受體(IL6R)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEB815Mu | 白介素6受體(IL6R)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB815Mu | 白介素6受體(IL6R)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| J Orthop Res | Interleukin‐6 and interleukin‐6 receptor expression, localization, and involvement in pain‐sensing neuron activation in a mouse intervertebral disc injury model [PubMed: 26010074] |
| Gene.? | The IL6R gene polymorphisms are associated with sIL-6R, IgE and lung function in Chinese patients with asthma [Pubmed:26997259] |
| Cytokine | Interleukin-6 participates in human pancreatic stellate cell activation and collagen I production via TGF-|?1/Smad pathway [33893003] |
