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尿激酶型纖溶酶原激活因子受體(uPAR)活性蛋白

Active Plasminogen Activator, Urokinase Receptor (uPAR)

CD87; PLAUR; PLAU-R; U-PAR; URKR; Monocyte Activation Antigen Mo3

  • 尿激酶型纖溶酶原激活因子受體(uPAR)活性蛋白 產(chǎn)品包裝(模擬)
  • 尿激酶型纖溶酶原激活因子受體(uPAR)活性蛋白 產(chǎn)品包裝(模擬)
  • APA141Mu01.jpg Figure. SDS-PAGE
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

Plasminogen Activator, Urokinase Receptor (uPAR) is the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. The urokinase receptor (uPAR) was identified as the membrane receptor of the serine protease urokinase (uPA), thereby implicated in the plasminogen activation cascade and regulation of pericellular proteolysis, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse uPAR and recombinant human uPA. Briefly, uPAR was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to uPA-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-uPAR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse uPAR and recombinant human uPA was shown in Figure 1, the EC50?for this effect is 0.069 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過一個(gè)月,-80°C不超過12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA141Mu01 尿激酶型纖溶酶原激活因子受體(uPAR)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA141Mu01 尿激酶型纖溶酶原激活因子受體(uPAR)活性蛋白 Cell?culture;?Activity?Assays.
RPA141Mu02 尿激酶型纖溶酶原激活因子受體(uPAR)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA141Mu01 尿激酶型纖溶酶原激活因子受體(uPAR)多克隆抗體 WB; IHC; ICC; IP.
PAA141Mu02 尿激酶型纖溶酶原激活因子受體(uPAR)多克隆抗體 WB; IHC; ICC; IP.
LAA141Mu71 尿激酶型纖溶酶原激活因子受體(uPAR)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
LAA141Mu81 尿激酶型纖溶酶原激活因子受體(uPAR)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
SEA141Mu 尿激酶型纖溶酶原激活因子受體(uPAR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA141Mu 尿激酶型纖溶酶原激活因子受體(uPAR)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
International Journal of Cancer Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice [Wiley: source]
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604]
Am J Emerg Med Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic guidance of sepsis [PubMed: 26615223]
Archives of Clinical Infectious Diseases Evaluation of Diagnostic Value of Soluble Urokinase-Type Plasminogen Activator Receptor in Sepsis [Archcid: 26346]
The American Journal of Emergency Medicine Clinical value of soluble urokinase-type plasminogen activator receptor in the diagnosis, prognosis, and therapeutic Cavia (Guinea pig )idance of sepsis [science:S0735675715009559]
Journal of Clinical and Experimental Hepatology Evaluation of Plasma Urokinase-Type Plasminogen Activator Receptor (UPAR) in Patients With Chronic Hepatitis B, C and Non-Alcoholic Fatty Liver Disease (NAFLD)?… [10.1016:j.jceh.2018.02.001]
Turkish journal of trauma & emergency surgery The diagnostic value of serum urokinase-type plasminogen activator receptor in acute appendicitis [Pubmed: 31475329]
CELL TRANSPLANTATION Predictive Value of Degranulating Factors of Neutrophils in Massive Cerebral Infarction [33787356]
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