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再生胰島衍生蛋白3β(REG3b)活性蛋白

Active Regenerating Islet Derived Protein 3 Beta (REG3b)

PAP1; Pancreatitis-Associated Protein 1

  • 再生胰島衍生蛋白3β(REG3b)活性蛋白 產(chǎn)品包裝(模擬)
  • 再生胰島衍生蛋白3β(REG3b)活性蛋白 產(chǎn)品包裝(模擬)
  • APK541Mu01.jpg SDS-PAGE圖
  • 再生胰島衍生蛋白3β(REG3b)活性蛋白 Figure. Western Blot; Sample: Recombinant REG3b, Mouse.
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實驗

Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM?prior to the addition of various concentrations of REG3b. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of REG3b was shown in Figure 2. It was obvious that REG3b significantly promoted cell proliferation of SK-N-SH cells. The ED50 for this effect is typically3.685-84.37ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲存

避免反復(fù)凍融。2-8°C不超過一個月,-80°C不超過12個月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。

相關(guān)產(chǎn)品

編號 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
APK541Mu01 再生胰島衍生蛋白3β(REG3b)活性蛋白 Cell?culture;?Activity?Assays.
RPK541Mu01 再生胰島衍生蛋白3β(REG3b)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPK541Mu02 再生胰島衍生蛋白3β(REG3b)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAK541Mu01 再生胰島衍生蛋白3β(REG3b)多克隆抗體 WB; IHC; ICC; IP.
PAK541Mu02 再生胰島衍生蛋白3β(REG3b)多克隆抗體 WB; IHC; ICC; IP.
SEK541Mu 再生胰島衍生蛋白3β(REG3b)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMK541Mu 再生胰島衍生蛋白3β(REG3b)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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