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Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)活性蛋白

Active T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT)

VSIG9; VSTM3; V-Set And Immunoglobulin Domain Containing 9; V-Set And Transmembrane Domain Containing 3

  • Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)活性蛋白 產(chǎn)品包裝(模擬)
  • Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)活性蛋白 產(chǎn)品包裝(模擬)
  • APN056Hu61.jpg Figure. SDS-PAGE
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

活性實(shí)驗(yàn)

T cell immunoreceptor with Ig and ITIM domains (TIGIT) is an inhibitory receptor expressed on several types of lymphocytes. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs. Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif. A 170 aa variant diverges after aa 166. Lymphocyte Activation Gene 3 (LAG3) and?TIGIT are two potential targets for cancer immunotherapy, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TIGIT and recombinant human LAG3. Briefly, biotin-linked TIGIT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LAG3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50 μl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human TIGIT and recombinant human LAG3 was shown in Figure 1, the EC50?for this effect is 0.11 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

儲(chǔ)存

避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月,-80°C不超過(guò)12個(gè)月。

穩(wěn)定性

熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。

相關(guān)產(chǎn)品

編號(hào) 適用物種:Homo sapiens (Human,人) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
APN056Hu61 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)活性蛋白 Cell?culture;?Activity?Assays.
RPN056Hu01 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPN056Hu02 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPN056Hu61 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAN056Hu01 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)多克隆抗體 WB
LAN056Hu81 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAN056Hu71 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAN056Hu22 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)單克隆抗體 WB; IHC; ICC; IP.
MAN056Hu23 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)單克隆抗體 WB; IHC; ICC; IP.
MAN056Hu24 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)單克隆抗體 WB; IHC; ICC; IP.
MAN056Hu21 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)單克隆抗體 WB
MAN056Hu25 Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)單克隆抗體 WB; IHC; ICC; IP.
SEN056Hu Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMN056Hu Ig和ITIM域蛋白T-細(xì)胞免疫受體(TIGIT)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
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