組織因子通道抑制因子2(TFPI2)活性蛋白
Active Tissue Factor Pathway Inhibitor 2 (TFPI2)
PP5; Placental protein 5
- 編號APB940Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點8.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Tissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 doesn’t have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2μg/mL was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 8% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant mouse TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel; if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel.The result was shown in figure 1.
As the figure 1 shown, MMP-2 can be inhibited by recombinant mouse TFPI2 at least 2.5μg/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB940Mu01 | 組織因子通道抑制因子2(TFPI2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB940Mu01 | 組織因子通道抑制因子2(TFPI2)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB940Mu01 | 組織因子通道抑制因子2(TFPI2)多克隆抗體 | WB; IHC; ICC; IP. |
| SEB940Mu | 組織因子通道抑制因子2(TFPI2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB940Mu | 組織因子通道抑制因子2(TFPI2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PGB940Mu01 | 組織因子通道抑制因子2(TFPI2)引物對 | PCR |
