轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白
Active Transferrin Receptor (TFR)
CD71; TFR1; P90; TFRC; T9; Trfr; sTfR; Transferrin receptor protein 1, serum form
- 編號APB171Hu62
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Transferrin Receptor (TFR) , also known as cluster of differentiation 71 (CD71), is a type II transmembrane glycoprotein that plays a crucial role in the transport of iron into the cell. It binds to Transferrin (TRF), a carrier protein that transports iron in the blood, and facilitates the uptake of iron through endocytosis. This process is essential for many biological functions, including DNA synthesis and cell growth.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TFR and recombinant chicken TRF. Briefly, biotin-linked TFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to TRF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of TFR and TRF was shown in Figure 1, the EC50?for this effect is 0.068ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB171Hu62 | 轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB171Hu61 | 轉(zhuǎn)鐵蛋白受體(TFR)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPB171Hu61 | 轉(zhuǎn)鐵蛋白受體(TFR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB171Hu01 | 轉(zhuǎn)鐵蛋白受體(TFR)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB171Hu62 | 轉(zhuǎn)鐵蛋白受體(TFR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB171Hu01 | 轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體 | WB; IHC |
| CAB171Hu01 | 抗轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體 | Loading Control of WB. |
| LAB171Hu81 | 轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB171Hu71 | 轉(zhuǎn)鐵蛋白受體(TFR)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| CAB171Hu24 | 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | Loading Control of WB. |
| MAB171Hu22 | 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | IHC |
| MAB171Hu26 | 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | WB |
| MAB171Hu23 | 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB171Hu24 | 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB171Hu25 | 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | WB |
| MAB171Hu21 | 轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | WB; IHC; ICC; IP. |
| CAB171Hu22 | 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | Loading Control of WB. |
| FAB171Hu02 | 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體 | Flow cytometry. |
| FAB171Hu86 | 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體(異硫氰酸熒光素標(biāo)記) | FCM |
| FAB171Hu42 | 抗轉(zhuǎn)鐵蛋白受體(TFR)單克隆抗體(藻紅素標(biāo)記) | FCM |
| SEB171Hu | 轉(zhuǎn)鐵蛋白受體(TFR)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB171Hu | 轉(zhuǎn)鐵蛋白受體(TFR)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| British Journal of Nutrition | Survey of anaemia and Helicobacter pylori infection in adolescent girls in Suihua, China and enhancement of iron intervention effects by H. pylori eradication [PubMed: 22004585] |
| Food Funct | The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699] |
| Physiological research | The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743] |
| Clinical and Experimental?Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502] |
| J Mol Cell Cardiol | Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233] |
