糖化血紅蛋白A1c(HbA1c)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Glycated Hemoglobin A1c (HbA1c)
Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc
- 編號CCA190Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍5.86-1,500μg/mL
- 靈敏度最小可檢測劑量小于等于2.44μg/mL.
- 樣本類型serum, plasma and erythrocyte lysates
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3175 ¥ 4536 ¥ 20412 ¥ 38556 ¥ 317520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測糖化血紅蛋白A1c(HbA1c)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的糖化血紅蛋白A1c(HbA1c)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-93 | 81 |
| EDTA plasma(n=5) | 83-93 | 88 |
| heparin plasma(n=5) | 83-91 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的糖化血紅蛋白A1c(HbA1c)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中糖化血紅蛋白A1c(HbA1c)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-91% | 85-93% | 88-102% | 81-103% |
| EDTA plasma(n=5) | 82-94% | 78-101% | 84-97% | 79-99% |
| heparin plasma(n=5) | 80-90% | 82-93% | 91-105% | 78-96% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分鐘；
7. 讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將糖化血紅蛋白A1c(HbA1c)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原（標(biāo)準(zhǔn)品或樣本），待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強(qiáng)度和樣品中的糖化血紅蛋白A1c(HbA1c)呈負(fù)相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPA190Hu01 | 糖化血紅蛋白A1c(HbA1c)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA190Hu01 | 糖化血紅蛋白A1c(HbA1c)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA190Hu71 | 糖化血紅蛋白A1c(HbA1c)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA190Hu21 | 糖化血紅蛋白A1c(HbA1c)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA190Hu22 | 糖化血紅蛋白A1c(HbA1c)單克隆抗體 | WB; IHC; ICC; IP. |
| CEA190Hu | 糖化血紅蛋白A1c(HbA1c)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA190Hu | 糖化血紅蛋白A1c(HbA1c)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA190Hu | 糖化血紅蛋白A1c(HbA1c)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA190Hu01 | 糖化血紅蛋白A1c(HbA1c)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| KSA190Hu11 | 糖化血紅蛋白A1c(HbA1c)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Molecular nutrition & food research | Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro [pubmed:28105758] |
| 33 | d-Ribose as a Contributor to Glycated Haemoglobin [pubmed:29033370] |
| Journal of Functional Foods | Hypoglycemic effect of Hypericum attenuatum Choisy extracts on type 2 diabetes by regulating glucolipid metabolism and modulating gut microbiota [] |
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| Journal of Occupational and Environmental?Hygiene | Stressors, allostatic load, and health outcomes among women hotel housekeepers: A pilot study [Pubmed: 30615593] |
| Cell?Reports | Parabacteroides distasonis Alleviates Obesity and Metabolic Dysfunctions via Production of Succinate and Secondary Bile Acids [Pubmed: 30605678] |
| Journal of Agricultural and Food Chemistry | Gymnemic acid alleviates type 2 diabetes mellitus and suppresses endoplasmic reticulum stress in vivo and in vitro [] |
| Journal of Clinical Medicine | 1H Nuclear Magnetic Resonance (NMR)-Based Cerebrospinal Fluid and Plasma Metabolomic Analysis in Type 2 Diabetic Patients and Risk Prediction for Diabetic?… [Pubmed: 31248127] |
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| Cell Reports | Activation of a Specific Gut Bacteroides-Folate-Liver Axis Benefits for the Alleviation of Nonalcoholic Hepatic Steatosis [Pubmed: 32783933] |
| The effects of different intensity endurance and resistance exercise on diabetic-related blood profiles in impaired glucose tolerance mice [] | |
| Revista Internacional de Andrologia | Relation of pharmacopenile duplex ultrasonography parameters and glycated hemoglobin (HbA1c) in diabetic patients with erectile dysfunction [33334711] |
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