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α-黑素細(xì)胞刺激素(aMSH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH)

α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin

  • α-黑素細(xì)胞刺激素(aMSH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • α-黑素細(xì)胞刺激素(aMSH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對α-黑素細(xì)胞刺激素(aMSH)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

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編號 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
CEA239Ra α-黑素細(xì)胞刺激素(aMSH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA239Ra α-黑素細(xì)胞刺激素(aMSH)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA239Ra11 α-黑素細(xì)胞刺激素(aMSH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
J Biochem. Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone. [Pubmed: 23341471]
Pigment Cell Melanoma Res Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by light [PubMed: 26582755]
The Journal of Biological Chemistry Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in Mice [Pubmed:27080257]
Pigment Cell & Melanoma Research Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by light [Pubmed:26582755]
Nature Communications Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives. [pubmed:28794470]
Cell?Stem?Cell Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses [Pubmed:29681516]
European Neuropsychopharmacology Epigenetic alterations of the POMC promoter in tobacco dependence [Pubmed:29871818]
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor [Pubmed:29685637]
Neuropsychobiology Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol Dependence [Pubmed: 31117084]
INTERNATIONAL IMMUNOPHARMACOLOGY Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathway [Pubmed: 32892076]
J Endocrinol Invest Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader?… [Pubmed:35098494]
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