上皮中性粒細胞激活肽78(ENA78)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Epithelial Neutrophil Activating Peptide 78 (ENA78)
CXCL5; SCYB5; Chemokine C-X-C-Motif Ligand 5; Small Inducible Cytokine Subfamily B(Cys-X-Cys),Member 5; Neutrophil-activating peptide ENA-78
- 編號KSA860Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍62.5-4000pg/mL
- 可適靈敏度25.7pg/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 6998 ¥ 11664 ¥ 20995 ¥ 40824 ¥ 69984
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對上皮中性粒細胞激活肽78(ENA78)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA860Mu02 | 上皮中性粒細胞激活肽78(ENA78)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA860Mu02 | 上皮中性粒細胞激活肽78(ENA78)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA860Mu61 | 上皮中性粒細胞激活肽78(ENA78)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA860Mu63 | 上皮中性粒細胞激活肽78(ENA78)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA860Mu61 | 上皮中性粒細胞激活肽78(ENA78)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA860Mu01 | 上皮中性粒細胞激活肽78(ENA78)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA860Mu02 | 上皮中性粒細胞激活肽78(ENA78)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA860Mu01 | 上皮中性粒細胞激活肽78(ENA78)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA860Mu81 | 上皮中性粒細胞激活肽78(ENA78)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAA860Mu71 | 上皮中性粒細胞激活肽78(ENA78)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA860Mu | 上皮中性粒細胞激活肽78(ENA78)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA860Mu | 抗上皮中性粒細胞激活肽78抗體(Anti-ENA78)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA860Mu | 上皮中性粒細胞激活肽78(ENA78)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA860Mu | 上皮中性粒細胞激活肽78(ENA78)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA860Mu01 | 上皮中性粒細胞激活肽78(ENA78)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Biochemical and Molecular Toxicology | Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis. [Pubmed:26862043] |
| Journal of Biochemical and Molecular Toxicology | Ameliorative Effects of Curcumin on Fibrinogen-Like Protein-2 Gene Expression, Some Oxido-Inflammatory and Apoptotic Markers in a Rat Model of l-Arginine-Induced Acute Pancreatitis [pubmed:26862043] |
| OncoTargets and Therapy | The clinical significance of CXCL5 in non-small cell lung cancer. [pubmed:29200871] |
| Biochimie | Activated CXCL5-CXCR2 axis promotes the migration, invasion and EMT of papillary thyroid carcinoma cells via modulation of β-catenin pathway [Pubmed:29471001] |
| Cancer?Biology?&?Therapy | Activation of CXCL5-CXCR2 axis promotes proliferation and accelerates G1 to S phase transition of papillary thyroid carcinoma cells and activates JNK and p38?… [Pubmed: 30404567] |
| Respiratory?Research | Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from?… [Pubmed: 30634975] |
| American Journal of Pathology | Interleukin17–CXCR2 axis facilitates breast cancer progression by up-regulating neutrophil recruitment [Pubmed: 31654638] |
| Bioactive Materials | Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy [34820587] |
| Am J Cancer Res | Differential expression profile of CXC-receptor-2 ligands as potential biomarkers in pancreatic ductal adenocarcinoma [Pubmed:35141005] |
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