脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Fatty Acid Binding Protein 4 (FABP4)
A-FABP; AFABP; AP2; Fatty Acid Binding Protein 4, Adipocyte; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein
- 編號(hào)KSB693Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍31.2-2000pg/mL
- 可適靈敏度13.8pg/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對(duì)脂肪細(xì)胞型脂肪酸結(jié)合蛋白(FABP4)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB693Mu02 | 脂肪酸結(jié)合蛋白 4(FABP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB693Mu01 | 脂肪酸結(jié)合蛋白 4(FABP4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB693Mu61 | 脂肪酸結(jié)合蛋白 4(FABP4)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB693Mu01 | 脂肪酸結(jié)合蛋白 4(FABP4)活性蛋白 | Cell?culture;?Activity?Assays. |
| APB693Mu61 | 脂肪酸結(jié)合蛋白 4(FABP4)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAB693Mu06 | 脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB693Mu02 | 脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB693Mu01 | 脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB693Mu71 | 脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAB693Mu81 | 脂肪酸結(jié)合蛋白 4(FABP4)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| SEB693Mu | 脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB693Mu | 脂肪酸結(jié)合蛋白 4(FABP4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB693Mu01 | 脂肪酸結(jié)合蛋白 4(FABP4)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clinical and Molecular Hepatology | Enhanced A-FABP expression in visceral fat: potential contributor to the progression of NASH [PubMed: 23091808] |
| journal of Human Hypertension | Siesta is associated with reduced systolic blood pressure level and decreased prevalence of hypertension in older adults [PubMed: 26134622] |
| genetics and molecular research | Overexpression of the A-FABP gene facilitates intermuscular fat deposition in transgenic mice [PubMed: 25867423] |
| Food Chem | Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytes [PubMed: 25952883] |
| Placenta | Increased expression of fatty acid binding protein 4 in preeclamptic Placenta and its relevance to preeclampsia [Pubmed:26992681] |
| Clin Chim Acta. | Valproate acid (VPA)-induced dysmetabolic function in clinical and animal studies. [pubmed:28161274] |
| Oxidative?Medicine?and?Cellular?Longevity | Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague?… [Pubmed:29849871] |
| Nutrition &?Diabetes | Lack of pronounced changes in the expression of fatty acid handling proteins in adipose tissue and plasma of morbidly obese humans [Pubmed:29335416] |
| American Journal of Cancer Research | 25-HC promotes hepatocellular carcinoma metastasis through up-regulation of TLR4 dependent FABP4 [Pubmed: 31720079] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074] |
| cells | The Effect of Mineralocorticoid Receptor 3 Antagonists on Anti-Inflammatory and Anti-Fatty Acid Transport Profile in Patients with Heart Failure [Pubmed:35455943] |
| Bone Research | FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis [Pubmed:35729106] |
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