賴氨酰氧化酶(LOX)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Lysyl Oxidase (LOX)
Protein-Lysine 6-Oxidase
- 編號KSC580Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.061ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7560 ¥ 12600 ¥ 22680 ¥ 44100 ¥ 75600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質量體系認證
特異性
試劑盒中的抗體對賴氨酰氧化酶(LOX)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| APC580Hu01 | 賴氨酰氧化酶(LOX)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC580Hu01 | 賴氨酰氧化酶(LOX)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC580Hu02 | 賴氨酰氧化酶(LOX)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC580Hu61 | 賴氨酰氧化酶(LOX)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC580Hu02 | 賴氨酰氧化酶(LOX)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC580Hu01 | 賴氨酰氧化酶(LOX)多克隆抗體 | WB; IHC; ICC; IP. |
| LAC580Hu81 | 賴氨酰氧化酶(LOX)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAC580Hu71 | 賴氨酰氧化酶(LOX)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAC580Hu22 | 賴氨酰氧化酶(LOX)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC580Hu | 賴氨酰氧化酶(LOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEC580Hu | 賴氨酰氧化酶(LOX)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC580Hu | 賴氨酰氧化酶(LOX)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSC580Hu01 | 賴氨酰氧化酶(LOX)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| American Journal of Hematology | The expression of lysyl-oxidase gene family members in myeloproliferative neoplasms. [PubMed: 23494965] |
| Current Eye Research | High Glucose Induced Differential Expression of Lysyl Oxidase and Its Isoform in ARPE-19 Cells [Pubmed: 22966818] |
| European Journal of Surgical Oncology | Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed: 24246612] |
| Vaccine | Copia autorizada por CDR [Ingentaconnect: Source] |
| Arthritis & Rheumatology | Lysyl oxidase is a potential biomarker of fibrosis in systemic sclerosis [Pubmed: 24574233] |
| Eur J Surg Oncol.? | Evaluation of serum lysyl oxidase as a blood test for colorectal cancer [Pubmed:Pmc4237001] |
| BMC Cancer. | Ionizing radiation induces tumor cell lysyl oxidase secretion [Pubmed:Pmc4223762] |
| DNA Cell Biol. | Lysyl Oxidase Genetic Variants Affect Gene Expression in Cervical Cancer [Pubmed:24945327] |
| scandinavian journal of immunology | Increased Serum Levels of the IL-33 Neutralizing sST2 in Limited Cutaneous Systemic Sclerosis [PubMed: 26095613] |
| Curr Eye Res | Correlation of Aqueous Humor Lysyl Oxidase Activity with TGF-? Levels and LOXL1 Genotype in Pseudoexfoliation [Pubmed:27116380] |
| journal of bone and mineral metabolism | LOX Fails to Substitute for RANKL in Osteoclastogenesis. [pubmed:27606829] |
| 38 | Blocking Surgically Induced Lysyl Oxidase Activity Reduces the Risk of Lung Metastases. [pubmed:28445728] |
| Molecular Medicine Reports | Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen?induced arthritis [pubmed:28901438] |
| Revista Espa?ola de Cardiología?(English?Edition) | A Global Assessment of Circulating Prolysyl Oxidase in Nonischemic Patients With Garden-variety Heart Failure With Preserved Ejection Fraction [Pubmed:29807761] |
| Molecular?Medicine?Reports | Lysyl oxidase inhibition via β-aminoproprionitrile hampers human umbilical vein endothelial cell angiogenesis and migration in vitro [Pubmed:29393489] |
| Thyroid | Lysyl Oxidase is a key player in BRAF/MAPK pathway-driven thyroid cancer aggressiveness [Pubmed: 30398411] |
| Am J Physiol Lung Cell Mol Physiol | Lysyl Oxidase Directly Contributes to Extracellular Matrix Production and Fibrosis in Systemic Sclerosis. [Pubmed: 33026236] |
| J Am Heart Assoc | Collagen Cross?\Linking Is Associated With Cardiac Remodeling in Hypertrophic Obstructive Cardiomyopathy [33356379] |
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