超氧化物歧化酶1(SOD1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Superoxide Dismutase 1 (SOD1)
ALS; ALS1; IPOA; Superoxide Dismutase 1, Soluble; Amyotrophic Lateral Sclerosis 1,Adult; Superoxide dismutase [Cu-Zn]
- 編號KSB960Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍78-5000pg/mL
- 可適靈敏度37pg/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7798 ¥ 12996 ¥ 23393 ¥ 45486 ¥ 77976
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對超氧化物歧化酶1(SOD1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB960Ra01 | 超氧化物歧化酶1(SOD1)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB960Ra01 | 超氧化物歧化酶1(SOD1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB960Ra01 | 超氧化物歧化酶1(SOD1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB960Ra81 | 超氧化物歧化酶1(SOD1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAB960Ra71 | 超氧化物歧化酶1(SOD1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB960Ra22 | 超氧化物歧化酶1(SOD1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB960Ra23 | 超氧化物歧化酶1(SOD1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB960Ra24 | 超氧化物歧化酶1(SOD1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB960Ra25 | 超氧化物歧化酶1(SOD1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB960Ra21 | 超氧化物歧化酶1(SOD1)單克隆抗體 | WB; IHC. |
| SEB960Ra | 超氧化物歧化酶1(SOD1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB960Ra | 超氧化物歧化酶1(SOD1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB960Ra01 | 超氧化物歧化酶1(SOD1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| AGE | Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closure [Pubmed:24443098] |
| Cardiovascular Diabetology | Diabetic cardiomyopathy is associated with defective myocellular copper regulation and both defects are rectified by divalent copper chelation [Pubmed:24927960] |
| Arch Oral Biol | Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetes [PubMed: 26143097] |
| Oxidative Medicine and Cellular Longevity | BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats [Journals: Omcl] |
| О Р И Г И Н А Л Ь Н Ы Е С Т А Т Ь И | КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИ [docs:225188] |
| Evidence-Based Complementary and Alternative Medicine | Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae [pmc:PMC4794592] |
| Free Radic Biol Med. | DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts. [Pubmed:26806551] |
| Adv Med Sci. | Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. [pubmed:28242484] |
| Iranian?Journal of?Kidney?Diseases | Clinical Characterization of Serum Docosahexaenoic Acid and Its Relationship With Inflammation Factors in Patients With Diabetic Nephropathy [Pubmed:29507271] |
| Journal of Integrative Medicine | Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats [Doi: 10.1016/j.joim.2018.12.002] |
| Metallomics | Restoration of myocellular copper-trafficking proteins and mitochondrial copper enzymes repairs cardiac function in rats with diabetes-evoked heart failure [Pubmed: 31821401] |
| JOURNAL OF PHARMACY AND PHARMACOLOGY | Grape seed extract improved the fertility-enhancing effect of atorvastatin in high-fat diet-induced testicular injury in rats: involvement of antioxidant and anti-apoptotic??- [33793875] |
| ACS Omega | SOD1 is a Possible Predictor of COVID-19 Progression as Revealed by Plasma Proteomics [34250342] |
| Korean J Physiol Pharmacol | Improved motility in the gastrointestinal tract of a postoperative ileus rat model with ilaprazole [34697261] |
| Pharmaceuticals | Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients [34832849] |
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