脊椎蛋白2(SPON2)重組蛋白
Recombinant Spondin 2 (SPON2)
DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1
- 編號(hào)RPF396Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱(chēng)，不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位分泌, 細(xì)胞外基質(zhì)
- 預(yù)測(cè)分子量26.7kDa
- 實(shí)際分子量33kDa(差異分析請(qǐng)參閱說(shuō)明書(shū))
- 片段與標(biāo)簽Val119~Val330 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)5.8
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPF396Mu01 | 脊椎蛋白2(SPON2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF396Mu02 | 脊椎蛋白2(SPON2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF396Mu01 | 脊椎蛋白2(SPON2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAF396Mu02 | 脊椎蛋白2(SPON2)多克隆抗體 | WB; IHC; ICC; IP. |
| SEF396Mu | 脊椎蛋白2(SPON2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF396Mu | 脊椎蛋白2(SPON2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The Journal of Urology | Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancer [Pubmed: 23665271] |
| Oxford Journals | SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASES [Content: 30] |
| Nephrology (Carlton) | Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic Nephropathy [PubMed: 25973958] |
| Oncogene | The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation [pubmed:28991232] |
| Endocrinology | Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular development [Pubmed: 30715257] |
| Journal of Cellular and Molecular Medicine | Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice [Pubmed: 32614521] |
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