信號(hào)素3A(SEMA3A)重組蛋白
Recombinant Semaphorin 3A (SEMA3A)
SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
- 編號(hào)RPL917Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來(lái)源原核表達(dá)
- 宿主E.coli
- 內(nèi)毒素水平<1.0EU/µg(LAL法測(cè)定)
- 亞細(xì)胞定位分泌
- 預(yù)測(cè)分子量17.6kDa
- 實(shí)際分子量17kDa(差異分析請(qǐng)參閱說(shuō)明書(shū))
- 片段與標(biāo)簽Arg31~Pro150 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)8.9
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應(yīng)用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請(qǐng)參見(jiàn)活性蛋白。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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SDS-PAGE圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APL917Ra01 | 信號(hào)素3A(SEMA3A)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPL917Ra01 | 信號(hào)素3A(SEMA3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAL917Ra01 | 信號(hào)素3A(SEMA3A)多克隆抗體 | WB; IHC; ICC; IP. |
| MAL917Ra21 | 信號(hào)素3A(SEMA3A)單克隆抗體 | WB; IHC; ICC; IP. |
| SEL917Ra | 信號(hào)素3A(SEMA3A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LML917Ra | 信號(hào)素3A(SEMA3A)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Arthritis Research & Therapy | Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus [Biomed: ar3881] |
| journal of invastigative dermatology | Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus [PubMed: 26121211] |
| Neurology | Neurofilament?light?chain?level?is a?weak?risk factor?for the?development?of?MS. [pubmed:27521440] |
| Development | miR-126-5p promotes retinal endothelial cell survival through SetD5 regulatio in neurons [pubmed:29180574] |
| A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells [] | |
| Life Sciences | Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. [Pubmed: 32603817] |
| ACS NANO | Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling [Pubmed: 32413258] |
| PLoS One | Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes [33905439] |
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