白介素1受體Ⅰ(IL1R1)活性蛋白
Active Interleukin 1 Receptor Type I (IL1R1)
CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha
- 編號(hào)APA066Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)7.8
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1080 ¥ 2700 ¥ 5400 ¥ 16200 ¥ 40500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant IL1R1, Human.
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the?interleukin-1 receptor?family, and is a receptor for interleukin 1 alpha (IL1a), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Thus, a binding ELISA assay was conducted to detect the association of IL1R1 with IL1a. Briefly, IL1R1 were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1a-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1a was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA066Hu03 | 白介素1受體Ⅰ(IL1R1)重組蛋白 | 8.35 |
| RPA066Hu02 | 白介素1受體Ⅰ(IL1R1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA066Hu01 | 白介素1受體Ⅰ(IL1R1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA066Hu01 | 白介素1受體Ⅰ(IL1R1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA066Hu02 | 白介素1受體Ⅰ(IL1R1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA066Hu03 | 白介素1受體Ⅰ(IL1R1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA066Hu01 | 白介素1受體Ⅰ(IL1R1)多克隆抗體 | WB,IHC; FCM |
| LAA066Hu71 | 白介素1受體Ⅰ(IL1R1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAA066Hu22 | 白介素1受體Ⅰ(IL1R1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA066Hu | 白介素1受體Ⅰ(IL1R1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA066Hu | 白介素1受體Ⅰ(IL1R1)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clinical & Experimental Immunology | In vivo extravasation induces expression of IL-1R1 in human neutrophils [Wiley: source] |
| PLos One | The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective Endocarditis [Plosone: 0031490] |
| Journal of Clinical Apheresis | Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel disease [PubMed: 22267087] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| 中國(guó)當(dāng)代兒科雜志 | IL1R1 基因多態(tài)性與兒童哮喘的相關(guān)性 [article:13877] |
| STEM CELLS AND DEVELOPMENT | IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways [Pubmed: 33107399] |
| Journal of Integrative Medicine | Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats [Pubmed:35643766] |
