白介素1受體Ⅰ(IL1R1)多克隆抗體
Polyclonal Antibody to Interleukin 1 Receptor Type I (IL1R1)
CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha
- 編號PAA066Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA066Hu01-白介素1受體Ⅰ(IL1R1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應用WB,IHC; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Esophagus cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: A431 cell
Primary Ab: 20μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant IL1R1, Human.
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Western Blot; Sample: Raji cell lysate
Primary Ab: 3μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Raji cell lysate;
Lane 2: IL1R1 knockout Raji cell lysate;
Predicted MW: 65kd
Observed MW: 65kd
Primary Ab: 3μg/ml Rabbit Anti-Human IL1R1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human IL1R1 Polyclonal Antibody (Catalog PAA066Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對IL1R1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別IL1R1。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA066Hu03 | 白介素1受體Ⅰ(IL1R1)重組蛋白 | 8.35 |
| RPA066Hu02 | 白介素1受體Ⅰ(IL1R1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA066Hu01 | 白介素1受體Ⅰ(IL1R1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA066Hu01 | 白介素1受體Ⅰ(IL1R1)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAA066Hu02 | 白介素1受體Ⅰ(IL1R1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA066Hu03 | 白介素1受體Ⅰ(IL1R1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA066Hu01 | 白介素1受體Ⅰ(IL1R1)多克隆抗體 | WB,IHC; FCM |
| LAA066Hu71 | 白介素1受體Ⅰ(IL1R1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAA066Hu22 | 白介素1受體Ⅰ(IL1R1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA066Hu | 白介素1受體Ⅰ(IL1R1)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA066Hu | 白介素1受體Ⅰ(IL1R1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Clinical & Experimental Immunology | In vivo extravasation induces expression of IL-1R1 in human neutrophils [Wiley: source] |
| PLos One | The Gene Expression Analysis of Blood Reveals S100A11 and AQP9 as Potential Biomarkers of Infective Endocarditis [Plosone: 0031490] |
| Journal of Clinical Apheresis | Kinetics of the soluble IL-1 receptor type I during treatment with an LCAP filter in patients with inflammatory bowel disease [PubMed: 22267087] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| 中國當代兒科雜志 | IL1R1 基因多態(tài)性與兒童哮喘的相關性 [article:13877] |
| STEM CELLS AND DEVELOPMENT | IL-1α regulates osteogenesis and osteoclastic activity of dental follicle cells via JNK and p38 MAPK pathways [Pubmed: 33107399] |
| Journal of Integrative Medicine | Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats [Pubmed:35643766] |
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