芐氯素1(BECN1)多克隆抗體
Polyclonal Antibody to Beclin 1 (BECN1)
ATG6; VPS30; Coiled-Coil,Myosin-Like BCL2 Interacting Protein; ATG6 Autophagy Related 6 Homolog
- 編號PAJ557Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPJ557Hu01-芐氯素1(BECN1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛), Ovis aries; Ovine (Sheep，綿羊)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 30μg/ml Rabbit Anti-Human BECN1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Pancreas Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human BECN1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Sample: Rat Cerebrum lysate Primary Ab: 0.1μg/ml Rabbit Anti-Human BECN1 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate; Lane3: Mouse Cerebrum lysate; Lane4: Canine Cerebrum lysate; Lane5: Bovine Cerebrum lysate; Lane6: Caprine Cerebrum lysate; Lane7: T-47D cell lysate<br/>Primary Ab: 0.3μg/ml Rabbit Anti-Human BECN1 Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Figure. Western Blot; Lane1: Human A431 Cells; Lane2: Human Hela Cells; Lane3: Porcine Brain Tissue.
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Knockout Varification: <br/>Lane 1: Wild-type MCF7 cell lysate; <br/>Lane 2: BECN1 knockout MCF7 cell lysate; <br/>Predicted MW: 52kDa <br/>Observed MW: 60kDa<br/>Primary Ab: 3μg/ml Rabbit Anti-Human BECN1 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Ovine Cerebrum lysate; Lane2: Bovine Cerebrum lysate; Lane3: MCF7 cell lysate; Lane4: A431 cell lysate;<br/>Primary Ab: 0.1μg/ml Rabbit Anti-Human BECN1 Antibody<br/>Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對BECN1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別BECN1。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPJ557Hu01 | 芐氯素1(BECN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ557Hu01 | 芐氯素1(BECN1)多克隆抗體 | WB; IHC |
| MAJ557Hu21 | 芐氯素1(BECN1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAJ557Hu22 | 芐氯素1(BECN1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAJ557Hu23 | 芐氯素1(BECN1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAJ557Hu24 | 芐氯素1(BECN1)單克隆抗體 | WB; IHC; ICC; IP. |
| MAJ557Hu25 | 芐氯素1(BECN1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEJ557Hu | 芐氯素1(BECN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ557Hu | 芐氯素1(BECN1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Rejuvenation Res. | Can Enhanced Autophagy be Associated with Human Longevity? Serum Levels of the Autophagy Biomarker Beclin-1 are Increased in Healthy Centenarians [Pubmed:25307612] |
| J Cosmet Dermatol | A peel-off facial mask comprising myoinositol and trehalose-loaded liposomes improves adult female acne by reducing local hyperandrogenism and activating autophagy [pubmed:28342238] |
| Tumor Biology | The novel CXCR4 antagonist, PRX177561, reduces tumor cell proliferation and accelerates cancer stem cell differentiation in glioblastoma preclinical models [pubmed:28639900] |
| 19 | Beclin1 circulating levels and accelerated ageing markers in COPD [?DOI:?10.1183/1393003.congress-2017.PA1018] |
| Cell?Death?&?Disease | Beclin1 circulating levels and accelerated aging markers in COPD [Pubmed:29402890] |
| Clinica Chimica Acta | A novel combined score of biomarkers in sputum may be an indicator for lung cancer: A pilot study [Doi: 10.1016/j.cca.2018.09.027] |
| International Journal of Clinical and Experimental Pathology | Serum concentration of autophagy-related protein 7 is correlated with non-alcoholic fatty liver disease in Chinese adults [] |
| EXPERIMENTAL AND THERAPEUTIC MEDICINE | Crosstalk between vitamin D axis, inflammation and host immunity mechanisms: A prospective study [33936265] |
| International Journal for Research in Applied Sciences and Biotechnology | Inhibition of HepG-2 Cells (Liver Cancer Cell Line) Viability by 3-Hydroxypyridine-2-Carboxaldehyde N (4)-Methylthiosemicarbazone [] |
| BIOMARKERS OF AUTOPHAGY AND ANTIVIRAL ROLE OF PLACENTA FOR PREDICTING PRETERM DELIVERY IN PATIENTS WITH PRETERM DELIVERY??- [] | |
| European Journal of Natural History | IDENTIFICATION OF FACTORS CONNECTED WITH AUTOPHAGY ACTIVITY UNDER CONDITIONS OF PARTIAL NUTRITIONAL DEPRIVATION IN MEN [] |
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