碳酸酐酶Ⅱ(CA2)多克隆抗體
Polyclonal Antibody to Carbonic Anhydrase II (CA2)
CA-II; CAII; Car2; CAC; Carbonate dehydratase II; Carbonic anhydrase C
- 編號PAA782Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 NPA782Ra01-碳酸酐酶Ⅱ(CA2)天然蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 616 ¥ 1436 ¥ 2052 ¥ 5130 ¥ 20520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Rat Kidney Tissue; Primary Ab: 10μg/ml Rabbit Anti-Rat CA2 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Stomach Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Rat CA2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Rat CA2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Stomach Tissue
Primary Ab: 20μg/ml Rabbit Anti-Rat CA2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: Hepa1-6 cell
Primary Ab: 20μg/ml Rabbit Anti-Rat CA2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant CA2, Rat.
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Western Blot; Sample: Mouse Heart lysate;
Primary Ab: 2μg/ml Rabbit Anti-Rat CA2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Serum;
Primary Ab: 2μg/ml Rabbit Anti-Rat CA2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Spleen lysate;
Primary Ab: 2μg/ml Rabbit Anti-Rat CA2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Serum;
Primary Ab: 2μg/ml Rabbit Anti-Rat CA2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Ovary lysate;
Primary Ab: 2μg/ml Rabbit Anti-Rat CA2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對CA2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CA2。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPA782Ra01 | 碳酸酐酶Ⅱ(CA2)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA782Ra02 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell?culture;?Activity?Assays. |
| APA782Ra01 | 碳酸酐酶Ⅱ(CA2)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA782Ra01 | 碳酸酐酶Ⅱ(CA2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA782Ra02 | 碳酸酐酶Ⅱ(CA2)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA782Ra01 | 碳酸酐酶Ⅱ(CA2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA782Ra71 | 碳酸酐酶Ⅱ(CA2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA782Ra | 碳酸酐酶Ⅱ(CA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA782Ra | 碳酸酐酶Ⅱ(CA2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA782Ra01 | 碳酸酐酶Ⅱ(CA2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Proteomics Clin Appl.? | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two-dimensional nano-liquid chromatography/nanoelectrospray ionization-mass spectrometry. [Pubmed:26970563] |
| PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
| Cell & Molecular Biology | Identification of potential breast cancer markers in nipple discharge by protein profile analysis using two‐dimensional nano‐liquid chromatography/nanoelectrospray … [doi:10.1002] |
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