組織蛋白酶D(CTSD)多克隆抗體
Polyclonal Antibody to Cathepsin D (CTSD)
CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis,Neuronal 10
- 編號(hào)PAB280Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB280Ra01-組織蛋白酶D(CTSD)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4, 含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 性狀液體
- 濃度500μg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 652 ¥ 1520 ¥ 2172 ¥ 5430 ¥ 21720
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Cerebrum Tissue Primary Ab: 10μg/ml Rabbit Anti-Rat CTSD Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Rat Cerebellum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat CTSD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat CTSD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat CTSD Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant CTSD, Rat.
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Western Blot; Sample: Lane1: Rat Spinal cord lysate; Lane2: Rat Lung lysate; Lane3: Rat Cerebrum lysate
Primary Ab: 2μg/mL Rabbit Anti-Rat CTSD Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Cerebrum lysate
Primary Ab: 0.1μg/ml Rabbit Anti-Rat CTSD Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)CTSD的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CTSD。
用法
免疫印跡：0.2-2μg/mL;1:250-2500
免疫組織化學(xué)：5-20μg/mL;1:25-100
免疫細(xì)胞化學(xué)：5-20μg/mL;1:25-100
最佳稀釋倍數(shù)最終由用戶決定。
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB280Ra01 | 組織蛋白酶D(CTSD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB280Ra03 | 組織蛋白酶D(CTSD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB280Ra02 | 組織蛋白酶D(CTSD)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB280Ra03 | 組織蛋白酶D(CTSD)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB280Ra02 | 組織蛋白酶D(CTSD)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB280Ra01 | 組織蛋白酶D(CTSD)多克隆抗體 | WB; IHC; ICC; IP. |
| FAB280Ra01 | 抗組織蛋白酶D(CTSD)多克隆抗體 | Flow cytometry. |
| SEB280Ra | 組織蛋白酶D(CTSD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB280Ra | 組織蛋白酶D(CTSD)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular Immunology | Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro [ScienceDirect: S0161589011008297] |
| 21 | Plasma Cathepsin D Levels: A Novel Tool to Predict Pediatric Hepatic Inflammation [PubMed: 25732418] |
| Scientific Reportes | Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention [pubmed:27922112] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
| JGH OPEN | Limited applicability of cathepsin D for the diagnosis and monitoring of non‐alcoholic steatohepatitis [] |
| Diabetologia | Plasma cathepsin D activity is negatively associated with hepatic insulin sensitivity in overweight and obese humans [Pubmed: 31690989] |
| Antioxidants | Iron and Advanced Glycation End Products: Emerging Role of Iron in Androgen Deficiency in Obesity [Pubmed: 32235809] |
| Peptides | LVV-hemorphin-7 (LVV-H7) plays a role in antinociception in a rat model of alcohol-induced pain disorders [33253777] |
| Cells | Identification of Cathepsin D as a Plasma Biomarker for Alzheimer's Disease [33445607] |
| J Chem Neuroanat | Analysis of silymarin-modulating effects against acrylamide-induced cerebellar damage in male rats: Biochemical and pathological markers [33965515] |
| Front Cell Infect Microbiol | PGRS Domain of Rv0297 of Mycobacterium tuberculosis Is Involved in Modulation of Macrophage Functions to Favor Bacterial Persistence [33042856] |
| Nutr Metab Cardiovasc Dis | Serum CathepsinD in pregnancy: relation with metabolic and inflammatory markers and effects of fish oils and probiotics [Pubmed:35304048] |
| Biosensors | A Comparison of Various Chips Used for the Manufacture of Biosensors Applied in Non-Fluidic Array SPRi, Based on the Example of Determination of Cathepsin D [Pubmed:35049649] |
