葡萄糖6磷酸異構酶(GPI)多克隆抗體
Polyclonal Antibody to Glucose 6 Phosphate Isomerase (GPI)
AMF; NLK; PHI; SA36; Phosphoglucose Isomerase; Autocrine motility factor; Neuroleukin; Phosphohexose isomerase; Sperm antigen 36
- 編號PAA725Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA725Mu01-葡萄糖6磷酸異構酶(GPI)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種-
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 583 ¥ 1361 ¥ 1944 ¥ 4860 ¥ 19440
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Mouse Vas Deferens Tissue; Primary Ab: 10μg/ml Rabbit Anti-Mouse GPI Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Mouse GPI Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse GPI Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Testis Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse GPI Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Mouse GPI Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GPI, Mouse.
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Western Blot; Sample: Mouse Prostate lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GPI Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GPI Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human A549 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GPI Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GPI Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對GPI的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別GPI。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA725Mu02 | 葡萄糖6磷酸異構酶(GPI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA725Mu01 | 葡萄糖6磷酸異構酶(GPI)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA725Mu01 | 葡萄糖6磷酸異構酶(GPI)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA725Mu02 | 葡萄糖6磷酸異構酶(GPI)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA725Mu | 葡萄糖6磷酸異構酶(GPI)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA725Mu | 葡萄糖6磷酸異構酶(GPI)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Oncology Reports | Hyperthermia reduces migration of osteosarcoma by suppression of autocrine motility factor [PubMed: 23027359] |
| Arthritis & Rheumatology | Bruton's tyrosine kinase deficiency inhibits autoimmune arthritis but fails to block immune complex‐mediated inflammatory arthritis [Pubmed:26945549] |
| Rheumatoid Arthritis | Bruton's Tyrosine Kinase Deficiency Inhibits Autoimmune Arthritis in Mice but Fails to Block Immune Complex–Mediated Inflammatory Arthritis [pubmed:26945549] |
| Infection?and?Immunity | The GPI transamidase complex subunit PbGPI16 of Plasmodium berghei is important for inducing experimental cerebral malaria [Pubmed:29784863] |
| Journal of Proteomics | Proteomics analysis of asthenozoospermia and identification of glucose-6-phosphate isomerase as an important enzyme for sperm motility [Pubmed: 31394311] |
| Scientific Reports | The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI … [Pubmed: 31959860] |
| JOURNAL OF IMMUNOLOGY | T–B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein [Pubmed: 33199538] |
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