血小板型磷酸果糖激酶(PFKP)多克隆抗體
Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
PFK-C; PFKF; ATP-dependent 6-phosphofructokinase, platelet type; 6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; Phosphohexokinase
- 編號PAB745Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB745Hu01-血小板型磷酸果糖激酶(PFKP)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 598 ¥ 1394 ¥ 1992 ¥ 4980 ¥ 19920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human PFKP Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Samples: Mouse Cardiac Muscle Tissue; <br/>Primary Ab: 10μg/ml Rabbit Anti-Human PFKP Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Cerebrum Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;<br/>Samples: Mouse Spleen Tissue; <br/>Primary Ab: 10μg/ml Rabbit Anti-Human PFKP Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;<br/>Sample: Hela cell<br/>Primary Ab: 20μg/ml Rabbit Anti-Human PFKP Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
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Western Blot; Sample: Lane1: Porcine Heart lysate; Lane2: Porcine Skeletal muscle lysate; Lane3: A549 cell lysate; Lane4: Jurkat cell lysate Primary Ab: 0.3μg/ml Rabbit Anti-Human PFKP Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
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Western Blot; Sample: Lane1: A549 cell lysate; Lane2: Porcine Heart lysate; Lane3: Jurkat cell lysate; Lane4: Porcine Skeletal muscle lysate<br/>Primary Ab: 3μg/ml Rabbit Anti-Human PFKP Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Knockout Varification:
Lane 1: Wild-type A549 cell lysate;
Lane 2: PFKP knockout A549 cell lysate;
Predicted MW: 85kd
Observed MW: 85kd
Primary Ab: 3μg/ml Rabbit Anti-Human PFKP Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human PFKP Polyclonal Antibody (Catalog PAB745Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對PFKP的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別PFKP。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB745Hu01 | 血小板型磷酸果糖激酶(PFKP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB745Hu01 | 血小板型磷酸果糖激酶(PFKP)多克隆抗體 | WB; IHC; FCM |
| MAB745Hu22 | 血小板型磷酸果糖激酶(PFKP)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB745Hu | 血小板型磷酸果糖激酶(PFKP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB745Hu | 血小板型磷酸果糖激酶(PFKP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| J Cell Mol Med | Huaier shows anti?\cancer activities by inhibition of cell growth, migration and energy metabolism in lung cancer through PI3K/AKT/HIF?\1|á pathway [33377619] |
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