肌動蛋白β(ACTB)單克隆抗體
Monoclonal Antibody to Beta Actin (ACTB)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- 編號MAB340Hu21
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C2
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB340Hu01-肌動蛋白β(ACTB)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 752 ¥ 1756 ¥ 2508 ¥ 6270 ¥ 25080
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 10μg/ml Mouse Anti-Human ACTb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Tonsil Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Tonsil Tissue
Primary Ab: 10μg/ml Mouse Anti-Human ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10μg/ml Mouse Anti-Human ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Human ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 30ug/ml Mouse Anti-Human ACTB Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Human ACTB Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Sample: Human Lung lysate; Primary Ab: 3μg/ml Mouse Anti-Human ACTb Antibody Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Hela cell lysate
Primary Ab: 0.1μg/ml Mouse Anti-Human IGF1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對ACTB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ACTB。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB340Hu01 | 肌動蛋白β(ACTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB340Hu01 | 肌動蛋白β(ACTB)多克隆抗體 | WB; IHC; ICC; IP. |
| CAB340Hu01 | 抗肌動蛋白β(ACTB)多克隆抗體 | Loading Control of WB. |
| MAB340Hu22 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Hu23 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Hu24 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Hu21 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Hu25 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| CAB340Hu22 | 抗肌動蛋白β(ACTB)單克隆抗體 | Loading Control of WB |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104] |
| European Journal of BioMedical Research | Expression of chloride channels in mouse skin incisional wounds [96] |
| Cell?Metabolism | Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes [] |
| Biological & Pharmaceutical Bulletin | Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine [Pubmed: 31902915] |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779] |
| JOURNAL OF INVESTIGATIVE MEDICINE | Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteries [Pubmed: 32784207] |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring [] |
| Genes | Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells [33261077] |
| Nat Commun | POLRMT mutations impair mitochondrial transcription causing neurological disease [33602924] |
| Life Sci | Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus [Pubmed:34990649] |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498] |
| Multimedia Tools and Applications | Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer [] |
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