肌動蛋白β(ACTB)單克隆抗體
Monoclonal Antibody to Beta Actin (ACTB)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- 編號MAB340Mi23
- 物種Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C6
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB340Mi01-肌動蛋白β(ACTB)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/ml
- 且適物種Oryctolagus cuniculus (Rabbit，兔)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 778 ¥ 1814 ¥ 2592 ¥ 6480 ¥ 25920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Tonsil Tissue; Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 30μg/ml Mouse Anti-Multi-species ACTB Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 30μg/ml Mouse Anti-Multi-species ACTB Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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Western Blot; Sample: HepG2 cell lysate Primary Ab: 0.2μg/ml Mouse Anti-Multi-species ACTB Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Porcine Kidney lysate; Lane3: Rabbit Kidney lysate; Lane4: Bovine Kidney lysate; Lane5: Canine Kidney lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對ACTB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ACTB。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB340Mi01 | 肌動蛋白β(ACTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAB340Mi01 | 抗肌動蛋白β(ACTB)多克隆抗體 | Loading Control of WB. |
| PAB340Mi01 | 肌動蛋白β(ACTB)多克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Mi22 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| CAB340Mi22 | 抗肌動蛋白β(ACTB)單克隆抗體 | Loading Control of WB |
| MAB340Mi23 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Mi21 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB340Mi24 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB340Mi | 肌動蛋白β(ACTB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB340Mi01 | 肌動蛋白β(ACTB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| PGB340Mi01 | 肌動蛋白β(ACTB)引物對 | PCR |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104] |
| European Journal of BioMedical Research | Expression of chloride channels in mouse skin incisional wounds [96] |
| Cell?Metabolism | Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes [] |
| Biological & Pharmaceutical Bulletin | Neuroinflammation, Oxidative Stress, and Neurogenesis in a Mouse Model of Chronic Fatigue Syndrome, and the Treatment with Kampo Medicine [Pubmed: 31902915] |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779] |
| JOURNAL OF INVESTIGATIVE MEDICINE | Effect of ischemic postconditioning on cell apoptosis and expression of relevant genes in non-culprit coronary arteries [Pubmed: 32784207] |
| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring [] |
| Genes | Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells [33261077] |
| Nat Commun | POLRMT mutations impair mitochondrial transcription causing neurological disease [33602924] |
| Life Sci | Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus [Pubmed:34990649] |
| J Ethnopharmacol | Isolation and characterization of anti-inflammatory and anti-proliferative compound, for B-cell Non-Hodgkin lymphoma, from Nyctanthes arbor-tristis Linn. [Pubmed:35398498] |
| Multimedia Tools and Applications | Investigation of single beam ultrasound sensitivity as a monitoring tool for local hyperthermia treatment in breast cancer [] |
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