CD73分子(CD73)單克隆抗體
Monoclonal Antibody to Cluster Of Differentiation 73 (CD73)
NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto
- 編號MAB250Hu23
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)D7
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPB250Hu01-CD73分子(CD73)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 652 ¥ 1520 ¥ 2172 ¥ 5430 ¥ 21720
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 10μg/ml Mouse Anti-Human CD73 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human CD73 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human CD73 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human CD73 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
AF488 staining on IF;
Sample: U87MG cell
Primary Ab: 30μg/ml Mouse Anti-Human CD73 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對CD73的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CD73。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB250Hu01 | CD73分子(CD73)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB250Hu01 | CD73分子(CD73)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB250Hu01 | CD73分子(CD73)多克隆抗體 | WB; IHC; ICC; IP. |
| FAB250Hu01 | 抗CD73分子(CD73)多克隆抗體 | Flow cytometry. |
| LAB250Hu81 | CD73分子(CD73)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| LAB250Hu71 | CD73分子(CD73)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| FAB250Hu41 | 抗CD73分子(CD73)多克隆抗體(藻紅素標記) | Flow cytometry. |
| FAB250Hu51 | 抗CD73分子(CD73)多克隆抗體(別藻藍蛋白標記) | Flow cytometry. |
| FAB250Hu81 | 抗CD73分子(CD73)多克隆抗體(異硫氰酸熒光素標記) | Flow cytometry. |
| RAB250Hu21 | CD73分子(CD73)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| MAB250Hu21 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB250Hu24 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB250Hu25 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB250Hu22 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB250Hu27 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB250Hu29 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB250Hu23 | CD73分子(CD73)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB250Hu | CD73分子(CD73)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB250Hu | CD73分子(CD73)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB250Hu01 | CD73分子(CD73)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Autoimmunity | The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation [Pubmed:25640206] |
| Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment?… [10.1016:j.talanta.2018.01.044] |
| Cancer?Biomarkers | Expression and clinical significance of serum NT5E protein in patients with colorectal cancer [Pubmed: 30932882] |
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