八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體
Monoclonal Antibody to Octamer Binding Transcription Factor 4 (OCT4)
POU5F1; OCT3; OTF3; OTF4; Octamer-4; POU Domain Class 5 Transcription Factor 1; POU Class 5 Homoeobox 1; Octamer-binding protein 3
- 編號(hào)MAA424Hu21
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C7
- Ig亞型 IgG2a Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPA424Hu01-八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.69mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 605 ¥ 1411 ¥ 2016 ¥ 5040 ¥ 20160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Liver Tissue Primary Ab: 30μg/ml Mouse Anti-Human OCT4 Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Rat Kidney Tissue
Primary Ab: 30μg/ml Mouse Anti-Human OCT4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue
Primary Ab: 30μg/ml Mouse Anti-Human OCT4 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
AF488 staining on IF;
Sample: HEL92.1.7 cell
Primary Ab: 20μg/ml Mouse Anti-Human OCT4 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu11)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)OCT4的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別OCT4。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA424Hu01 | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA424Hu01 | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA424Hu22 | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA424Hu23 | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA424Hu24 | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA424Hu21 | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA424Hu06 | 抗八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體 | Flow cytometry. |
| FAA424Hu04 | 抗八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體 | Flow cytometry. |
| FAA424Hu02 | 抗八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)單克隆抗體 | Flow cytometry. |
| SEA424Hu | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA424Hu | 八聚體結(jié)合轉(zhuǎn)錄因子4(OCT4)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Analytical Methods | A universal glucometer-based biosensor for portable and quantitative detection of transcription factors [Rsc: Source] |
| Oncology?Letters | Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma [Doi: 10.3892/ol.2018.9381] |
| Stem Cell Research | Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4+ T cells of healthy individuals of Indian origin [] |
| 博士論文 | Experimental and Modeling Framework for the Production of Cell and Protein-Based Biopharmaceutical Products [] |
| Stem Cell Research | Generation and characterization of three clones (NCCSi007A, NCCSi007B and NCCSi007C) of an integration free induced Pluripotent Stem Cell line from a?… [Pubmed: 31862609] |
| J Biochem Mol Toxicol | Studies on male gonadal toxicity of bisphenol A in diabetic rats: An example of exacerbation effect [Pubmed:35187753] |
