微管蛋白β(TUBb)單克隆抗體
Monoclonal Antibody to Tubulin Beta (TUBb)
TUB-B; TUBB5; TUBB1; M40
- 編號(hào)MAB870Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來(lái)源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號(hào))C1
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPB870Hu01-微管蛋白β(TUBb)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Cavia (Guinea pig ，豚鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，綿羊), Chicken (Gallus，雞)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 778 ¥ 1814 ¥ 2592 ¥ 6480 ¥ 25920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Endometrial cancer Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Prostate Tissue;
Primary Ab: 10μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Samples: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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WB圖
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Cavia Kidney lysate; Lane4: Canine Kidney lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Kidney lysate; Lane7: Rabbit Cerebrum lysate; Lane8: Rabbit Kidney lysate; Lane9: Caprine Kidney lysate
Primary Ab: 1μg/ml Mouse Anti-Human TUBb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Human SH-SY5Y cell line was stained with 10μg/ml mouse Anti-human TUBb Monoclonal Antibody (Catalog MAB870Hu22, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1μg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)TUBb的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別TUBb。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry: 10μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APB870Hu01 | 微管蛋白β(TUBb)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPB870Hu01 | 微管蛋白β(TUBb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CAB870Hu01 | 抗微管蛋白β(TUBb)多克隆抗體 | Loading Control of WB. |
| PAB870Hu01 | 微管蛋白β(TUBb)多克隆抗體 | WB; IHC; ICC/IF |
| MAB870Hu22 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB870Hu23 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB870Hu25 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB870Hu27 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB870Hu24 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB870Hu21 | 微管蛋白β(TUBb)單克隆抗體 | WB; IHC; ICC; IP. |
| CAB870Hu22 | 抗微管蛋白β(TUBb)單克隆抗體 | Loading Control of WB. |
| FAB870Hu06 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
| FAB870Hu04 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
| FAB870Hu02 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
| FAB870Hu08 | 抗微管蛋白β(TUBb)單克隆抗體 | Flow cytometry. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| archives of pharmacal research | Synthesis and cytotoxic activity of certain trisubstituted azetidin-2-one derivatives as a cis-restricted combretastatin A-4 analogues [pubmed:27747473] |
| archiv der pharmazie (Weinheim). | New 3-Substituted-2-(4-hydroxyanilino)pyridine Derivatives: Synthesis, Antitumor Activity, and Tubulin Polymerization Inhibition. [pubmed:28150327] |
| ACS?Applied?Materials?&?Interfaces | Novel Beta-Tubulin-Immobilized Nanoparticles Affinity Material for Screening β-Tubulin Inhibitors from a Complex Mixture [pubmed:28112513] |
| Future?Medicinal Chemistry | Design, synthesis and biological evaluation of chromenopyrimidines as potential cytotoxic agents [Pubmed:29779400] |
| Future?Medicinal Chemistry | Design and synthesis of novel 5-(4-chlorophenyl) furan derivatives with inhibitory activity on tubulin polymerization [Pubmed:29966433] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167] |
| Journal?of?Enzyme?Inhibition?and?Medicinal?Chemistry | Design, synthesis, and screening of amino thiophene carboxamide derivatives on hepatocellular carcinomaas VEGFR-2Inhibitors [Pubmed: 30191744] |
| European Journal of Medicinal Chemistry | Potent combretastatin A-4 analogs containing 1, 2, 4-triazole: Synthesis, antiproliferative, anti-tubulin activity, and docking study [Pubmed: 31536891] |
| ACS Sensors | Early diagnosis of Alzheimer's disease in blood using a disposable electrochemical microfluidic platform [Pubmed: 32207606] |
| Bioorganic Chemistry | Tubulin Inhibitors: Discovery of a New Scaffold Targeting Extra-binding Residues within the Colchicine Site through Anchoring Substituents Properly Adapted to their?… [Pubmed: 32325332] |
| BRITISH JOURNAL OF NUTRITION | Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats [Pubmed: 33028437] |
| European Journal of Medicinal Chemistry | Design, Synthesis and Screening of Benzimidazole Containing Compounds with Methoxylated Aryl Radicals as Cytotoxic Molecules on (HCT-116) Colon?… [Pubmed: 33158579] |
| J BIOMOL STRUCT DYN/JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS | Cytotoxicity, in silico predictions and molecular studies for androstane heterocycle compounds revealed potential antitumor agent against lung cancer cells [33300466] |
| Cancer Cell International | Bufalin Induced Mitochondrial Dysfunction Promotes Apoptosis of Glioma Cells by Regulating Annexin A2 and DRP1 Proteins [] |
| Cancer Cell Int | Bufalin induces mitochondrial dysfunction and promotes apoptosis of glioma cells by regulating Annexin A2 and DRP1 protein expression [34376212] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026] |
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