輸出蛋白1(XPO1)多克隆抗體
Polyclonal Antibody to Exportin 1 (XPO1)
Exp1; CRM1 Homolog,Yeast; Chromosome region maintenance 1 protein homolog
- 編號PAC258Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC258Hu01-輸出蛋白1(XPO1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Uterus Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lymph node Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Pancreas Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Spinal cord Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant XPo1, Human.
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Western Blot; Sample: Lane1: Hela cell lysate; Lane2: K562 cell lysate; Lane3: Human Lung lysate; Lane4: Porcine Small intestine lysate
Primary Ab: 1μg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: XPo1 knockout Hela cell lysate;
Predicted MW: 123kd
Observed MW: 110kd
Primary Ab: 1μg/ml Rabbit Anti-Human XPO1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human XPo1 Polyclonal Antibody (Catalog PAC664Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對XPO1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別XPO1。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC258Hu01 | 輸出蛋白1(XPO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC258Hu01 | 輸出蛋白1(XPO1)多克隆抗體 | WB; IHC; FCM |
| FAC258Hu01 | 抗輸出蛋白1(XPO1)多克隆抗體 | Flow cytometry. |
| FAC258Hu51 | 抗輸出蛋白1(XPO1)多克隆抗體(別藻藍(lán)蛋白標(biāo)記) | Flow cytometry. |
| MAC258Hu21 | 輸出蛋白1(XPO1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC258Hu | 輸出蛋白1(XPO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC258Hu | 輸出蛋白1(XPO1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clin Cancer Res | Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402] |
| Clin Cancer Res | Inactivation of the p53–KLF8–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402] |
| Cellular Oncology | Mahendra Seervi, S. Sumi, Aneesh Chandrasekharan, Abhay K. Sharma & [Doi: 10.1007/s13402-019-00451-1] |
| Cellular Oncology | Molecular profiling of anastatic cancer cells: potential role of the nuclear export pathway [Pubmed: 31147963] |
| Journal of Cardiovascular Translational Research | XPO1 Gene Therapy Attenuates Cardiac Dysfunction in Rats with Chronic Induced Myocardial Infarction [Pubmed: 31768947] |
| Biol Chem | Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry [34472763] |
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